Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating AHR/NRF2/NLRP3 Inflammasome Pathways through Indole-3-lactic Acid Production
Qingyu Cui, Zhe Zhang, Xiaoying Tian, Xi Liang, Youyou Lu, Yixin Shi, Malina Kuerman, Rui Wang, Yu zhou, Pimin Gong, Kai Lin, Huaxi Yi, Jiadong Li, Tongjie Liu, Lanwei Zhang
Journal：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
IF：6.1
DOI：10.1021/acs.jafc.2c06894
PMID：36633059
Published：2023-01-12
research field：药剂学免疫学药物递送胃肠病学纳米医学
Abstract
In this study, the effectors and mechanisms of Bifidobacterium bifidum FL-276.1 and B. bifidum FL-228.1 in alleviating dextran sulfate sodium (DSS)-induced colitis were investigated. Both FL-276.1 and FL-228.1 significantly alleviated DSS-induced colitis, whether they were supplemented from the beginning of the experiment (whole course intervention) or after the DSS induction started (partial intervention). Aryl hydrocarbon receptor (AHR) and the nuclear factor erythroid 2-related factor 2 (NRF2) pathways were activated in mice colons, while the NLR family pyrin domain containing 3 (NLRP3) was downregulated under the whole course intervention modes. Indole-3-lactic acid, an AHR ligand produced by FL-276.1 and FL-228.1, could regulate the AHR/NRF2/NLRP3 pathway in Caco-2 monolayers, thus upregulating the tight junction proteins and protecting the integrity of the epithelial barrier. These results are conducive to promoting clinical trials and product development of probiotics for alleviating colitis.
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