Antifungal mechanisms of silver nanoparticles on mycotoxin producing rice false smut fungus
Hui Wen, Huanbin Shi, Nan Jiang, Jiehua Qiu, Fucheng Lin, Yanjun Kou
Journal：iScience
IF：6.11
DOI：10.1016/j.isci.2022.105763
PMID：36582831
Published：2022-12-08
research field：分子生物学真菌遗传学纳米技术植物病理学
Abstract
Summary Ustilaginoidea virens , which causes rice false smut disease, is a destructive filamentous fungal pathogen, attracting more attention to search for effective fungicides against U . virens . Here, the results showed that the inhibition of 2 nm AgNPs on U . virens growth and virulence displayed concentration-dependent manner. Abnormalities of fungal morphology were observed upon exposure to AgNPs. RNA-sequencing (RNA-seq) analysis revealed that AgNPs treatment up-regulated 1185 genes and down-regulated 937 genes, which significantly overlapped with the methyltransferase UvKmt6-regulated genes. Furthermore, we found that AgNPs reduced the UvKmt6-mediated H3K27me3 modification, resulting in the up-regulation of ustilaginoidin biosynthetic genes The decrease of H3K27me3 level was associated with the inhibition of mycelial growth by AgNPs treatment. These results suggested that AgNPs are an effective nano-fungicide for the control of rice false smut disease, but when using AgNPs, it needs to be combined with mycotoxin-reducing fungicides to reduce the risk of toxin pollution.
本文使用的Yeasen产品
