NDR1/HIN1-like genes may regulate Glycine max seed germination under chilling stress through the ABA pathway
Wang Jian, Wu Rong, Shangguan Tengwei, Chen Guang, Zheng Yuanting, Tao Xiaoyuan, Li Sujuan, Wang Yang, Xu Shengchun
Journal：PLANT GROWTH REGULATION
IF：3.24
DOI：10.1007/s10725-022-00894-x
PMID：
Published：2022-08-24
research field：药理学细胞生物学免疫学微生物学
Abstract
NDR1 / HIN1 - like ( NHL ) genes play essential roles in triggering plant defenses in response to environmental stimuli. In the present study, we performed comprehensive identification, characterization, and transcription analysis of NHL genes in Glycine max under chilling stress. Phylogenetic analysis showed that the 36 GmNHL s could be divided into 4 subgroups. Cis -element analysis revealed that the majority of their promoter regions contain phytohormone-responsive and abiotic stress-responsive elements, indicating their potential functions in stress responses. Germination analysis of soybean seeds resulted in detection of significantly delayed germination under chilling stress (10 ℃) and with abscisic acid (ABA, 15 μM) treatment; transcription analysis revealed GmNHL s to be differentially regulated in response to chilling stress and ABA treatment. In particular, NP_001239877.1 and XP_003532975.1 were significantly up-regulated, indicating a potential role of GmNHL s in mediating the crosstalk of responses to these treatments. In addition, key ABA biosynthesis genes and signal transduction genes were up-regulated under chilling stress. Therefore, we suggest that GmNHL s might be involved in regulating seed germination under chilling stress through the ABA regulation pathway.
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