The establishment of a GPD1L knockout human embryonic stem cell line (WAe009-A-80) using the CRISPR/Cas9 system
Tao Dong, Yan Zhao, Hai-feng Jin, Hong-ming Pan, Li-ling Yue, Yan Lin, Lei Shen
Journal：Stem Cell Research
IF：0.6
DOI：10.1016/j.scr.2026.103910
PMID：
Published：2026-01-08
research field：酶工程合成生物学真菌学天然产物化学生物化学
Abstract
The GPD1L gene is located on 3p22.3. It encodes the glycerol phosphate dehydrogenase 1-like protein with homology to glycerol phosphate dehydrogenase (GPD1L), but the function of this enzyme is unclear. Mutations in GPD1L have been associated with BrS (Brugada syndrome) and SIDS (sudden infant death syndrome) and reduce Na + inward current through an unknown mechanism in human cardiomyocytes. Here, a GPD1L knockout human embryonic stem cell line was generated using CRISPR/Cas9 system. The GPD1L knockout human embryonic stem cell maintains the pluripotency, differentiation into three germ layers, forming normal EBs.
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