Engineering catalytic dephosphorylation reaction for endotoxin inactivation
Meng Gao, Xi Liu, Zhenzhen Wang, Hui Wang, Tristan Asset, Di Wu, Jun Jiang, Qianqian Xie, Shujuan Xu, Xiaoming Cai, Jia Li, Weili Wang, Huizhen Zheng, Xingfa Gao, Nikolai Tarasenko, Benjamin Rotonnel
Journal：Nano Today
IF：18.96
DOI：10.1016/j.nantod.2022.101456
PMID：
Published：2022-03-14
research field：污染控制环境科学酶技术生物修复抗生素去除
Abstract
Although lives have been saved due to the discovery of endotoxin removal methods including solvent extraction and affinity adsorption, they have limitations in treatment capacity, efficiency or costs. Endotoxin contaminations still result in a large number of deaths in global every year. This necessitates a mechanistic breakthrough for endotoxin removal or inactivation. Herein, we engineered a dephosphorylation reaction on endotoxins by a synthetic nanozyme (CeO 2 ) to attenuate the toxicity. CeO 2 prepared in phosphate-free hydrothermal reaction selectively and efficiently catalyzed the breaking of P-O bonds in endotoxins. Catalytic depletion of phosphates from endotoxins attenuated their binding with Toll-like receptors, NF-κB activation and pro-inflammatory cytokine release. Airborne LPS was, for the first time, inactivated (98%) by this facile dephosphorylation reaction. A CeO 2 integrating column displayed a 16-fold higher treatment capacity than commercial resins to aqueous endotoxins (water and protein solution). Overall, our findings offer a different mechanistic insight for removal of endotoxins.
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