Ambient air PM2.5 exposure induces heart injury and cardiac hypertrophy in rats through regulation of miR-208a/b, α/β-MHC, and GATA4
Lirong Bai, Yufei Zhao, Lifang Zhao, Mei Zhang, Zongwei Cai, Ken Kin Lam Yung, Chuan Dong, Ruijin Li
Journal：ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
IF：4.86
DOI：10.1016/j.etap.2021.103653
PMID：33812011
Published：2021-03-31
research field：分子生物学转化医学肿瘤免疫学免疫治疗RNA生物学
Abstract
Ambient air fine particulate matter (PM 2.5 ) may increase cardiovascular disease risks. In this study, we investigated the miR-208/GATA4/myosin heavy chain (MHC) regulation mechanisms on cardiac injury in rats after PM 2.5 exposure via an animal inhalation device. The results showed that PM 2.5 exposure for 2 months caused pathological heart injury, reduced nucleus-cytoplasm ratio, and increased the levels of CK-MB and cTnI, showing cardiac hypertrophy. Oxidative stress and inflammatory responses were also observed in rats’ hearts exposed to PM 2.5 . Of note, PM 2.5 exposure for 2-month significantly elevated GATA4 and β-MHC mRNA and protein expression compared with the corresponding controls, along with the high-expression of miR-208b. The ratios of β-MHC/α-MHC expression induced by PM 2.5 were remarkably raised in comparison to their controls. It suggested that the up-regulation of miR-208b/β-MHC and GATA4 and the conversion from α-MHC to β-MHC may be the important causes of cardiac hypertrophy in rats incurred by PM 2.5 .
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