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Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 （peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

Western

Western blot analysis of Calreticulin in (1) U87-MG lysates; (2) A431 lysates using PBR antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human colon using PBR antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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