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The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  ELISA: 1/10000

Western

Western blot analysis of CDK1 in  Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate using CDK1 antibody.

Immunocytochemistry

Immunocytochemistry analysis of CDK1 in  Eca109 cells using CDK1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

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