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Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200

Western

Western blot analysis of Phospho-SMC1 (S957) in (1) HeLa lysates; (2) Hela lysates treated with AP using Phospho-SMC1A (Ser957) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Phospho-SMC1 (S957) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。