Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100
Western blot analysis of Phospho-IRE1 (Ser724) in HeLa lysates using Phospho-IRE1alphaS724 antibody.HeLa were treated by Calyculin A for 30 minutes after serumstarvation overnight
Western blot analysis of Phospho-IRE1 (Ser724) in NIH/3T3 lysates using Phospho-IRE1alphaS724 antibody.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Phospho-IRE1alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Phospho-IRE1alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。
