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The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

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Flow cytometric analysis of Histone acetyltransferase 1 expression in HeLa cells using Histone acetyltransferase 1 antibody . Green, isotype control; red, Histone acetyltransferase 1.

Immunocytochemical staining of Hela cells with Histone acetyltransferase 1 antibody . Nuclei were stained blue with DAPI; Histone acetyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Histone acetyltransferase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone acetyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Histone acetyltransferase 1 antibody . Histone acetyltransferase 1 expression in wild type (WT) and histone acetyltransferase 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Histone acetyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Histone acetyltransferase 1 antibody , Top panel: wild-type (WT); Bottom panal:Histone acetyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Histone acetyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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