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The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

推荐稀释比 WB: 1/1000-1/2500; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Casein kinase 1 epsilon expression in HT-1080 cells using Casein kinase 1 epsilon antibody . Green, isotype control; red, Casein kinase 1 epsilon.

Western blotting analysis using casein kinase 1 epsilon antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with casein kinase 1 epsilon antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using casein kinase 1 epsilon antibody . Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with casein kinase 1 epsilon antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Casein kinase 1 epsilon knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Casein kinase 1 epsilon antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Casein kinase 1 epsilon antibody , Top panel: wild-type (WT); Bottom panal: Casein kinase 1 epsilon shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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