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This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Protein arginine methyltransferase 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein arginine methyltransferase 7 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Protein arginine methyltransferase 7 expression in HepG2 cells using Protein arginine methyltransferase 7 antibody . Green, isotype control; red, Protein arginine methyltransferase 7.

Immunocytochemical staining of HepG2 cells with Protein arginine methyltransferase 7 antibody . Nuclei were stained blue with DAPI; Protein arginine methyltransferase 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Protein arginine methyltransferase 7 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Protein arginine methyltransferase 7 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Protein arginine methyltransferase 7 antibody . Protein arginine methyltransferase 7 expression in wild type (WT) and protein arginine methyltransferase 7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Protein arginine methyltransferase 7 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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