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The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Validation of NDUFS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with NDUFS1 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of NDUFS1 expression in HepG2 cells using NDUFS1 antibody . Green, isotype control; red, NDUFS1.

Immunocytochemical staining of HepG2 cells with NDUFS1 antibody . Nuclei were stained blue with DAPI; NDUFS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using NDUFS1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using NDUFS1 antibody . NDUFS1 expression in wild-type (WT) and NDUFS1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFS1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using NDUFS1 antibody , Top panel: wild-type (WT); Bottom panal: NDUFS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NDUFS1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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