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This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Exo/Endonuclease G knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Exo/Endonuclease G antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of exo/endonuclease G expression in HepG2 cells using exo/endonuclease G antibody . Green, isotype control; red, exo/endonuclease G.

Immunocytochemical staining of HepG2 cells with Exo/Endonuclease G antibody . Nuclei were stained blue with DAPI; Exo/Endonuclease G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Exo/Endonuclease G antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Exo/Endonuclease G antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Exo/endonuclease G antibody . Exo/Endonuclease G expression in wild type (WT) and exo/endonuclease G shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Exo/endonuclease G antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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