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This gene encodes a component of the motor complex, cytoplasmic dynein, which transports cellular cargo along microtubules in the cell. The encoded protein regulates the length of primary cilia which are sensory organelles found on the surface of cells. The protein encoded by this gene interacts with viral proteins, like the minor capsid protein L2 of human papillomavirus, and is required for dynein-mediated delivery of the viral nucleic acid to the host nucleus. This protein interacts with oncogenic nucleoporins to disrupt gene regulation and cause leukemic transformation. Pseudogenes of this gene are present on chromosomes 4 and 17. Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of DYNLT1 expression in HT-1080 cells using DYNLT1 antibody . Green, isotype control; red, DYNLT1.

Western blotting analysis using DYNLT1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNLT1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using DYNLT1 antibody . DYNLT1 expression in wild-type (WT) and DYNLT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DYNLT1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of DYNLT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DYNLT1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using DYNLT1 antibody , Top panel: wild-type (WT); Bottom panal: DYNLT1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DYNLT1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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