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This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of NAMPT expression in HepG2 cells using NAMPT antibody . Green, isotype control; red, NAMPT.

Immunocytochemical staining of HepG2 cells with NAMPT antibody . Nuclei were stained blue with DAPI; NAMPT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using NAMPT antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAMPT antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using NAMPT antibody . NAMPT expression in wild-type (WT) and NAMPT shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAMPT antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of NAMPT knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NAMPT antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using NAMPT antibody , Top panel: wild-type (WT); Bottom panal: NAMPT shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAMPT was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using NAMPT antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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