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This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.

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Flow cytometric analysis of Lysine demethylase 1A expression in HepG2 cells using Lysine demethylase 1A antibody . Green, isotype control; red, Lysine demethylase 1A.

Immunocytochemical staining of HepG2 cells with Lysine demethylase 1A antibody . Nuclei were stained blue with DAPI; Lysine demethylase 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using lysine demethylase 1A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using lysine demethylase 1A antibody . Lysine demethylase 1A expression in wild-type (WT) and lysine demethylase 1A (KDM1A) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lysine demethylase 1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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