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This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/50-1/100

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Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using Phospho-Vimentin (Ser72) antibody . Green, isotype control; red, Phospho-Vimentin (Ser72).

Immunocytochemical staining of HT-1080 cells with Phospho-Vimentin (Ser72) antibody . Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using phospho-vimentin (Ser72) antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using phospho-vimentin (Ser72) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-vimentin (Ser72) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-vimentin (Ser72) antibody . Phospho-vimentin (Ser72) expression in wild-type (WT) and VIM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-vimentin (Ser72) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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