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Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

推荐稀释比 WB: 1/4000-1/20000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Histone deacetylase 6 expression in HepG2 cells using Histone deacetylase 6 antibody . Green, isotype control; red, Histone deacetylase 6.

Immunocytochemical staining of HepG2 cells with Histone deacetylase 6 antibody . Nuclei were stained blue with DAPI; Histone deacetylase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Histone deacetylase 6 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone deacetylase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Histone deacetylase 6 antibody . Histone deacetylase 6 expression in wild type (WT) and Histone deacetylase 6 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Histone deacetylase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Histone deacetylase 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT1080 cells were stained with Histone deacetylase 6 antibody and analyzed using BD flow cytometer.

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