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Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  IP: 1/50  

Western blot

Western blot analysis of Caspase9 in HeLa lysates using Caspase 9 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human liver using Caspase9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Caspase 9 in HeLa treated with staurosporine using Caspase9 antibody.

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