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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

推荐稀释比 WB: 1/500-1/1000; IHC-P: 1/50-1/200; IF: 1/50-1/200

Western

Western blot analysis of TriMethyl-Histone H3 (Lys4) in various cell lines lysates using TriMethyl-Histone H3-K4 antibody.

TriMethyl-Histone

TriMethyl-Histone H3 (Lys4) D198 Dotblot analysis of all sorts of methylation peptides using TriMethyl-Histone H3 (Lys4) antibody

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human rectal cancer using TriMethyl-Histone H3-K4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of TriMethyl-Histone H3 (Lys4) in 293T using TriMethyl-Histone H3-K4 antibody,and DAPI (blue).

Chromatin Immunoprecipitation TriMethyl-Histone H3-K4 pAb Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input.

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