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These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

推荐稀释比 WB: 1/500-1/1000  IP: 1/50

Western

Western blot analysis of Phospho-Chk2 (T68) in (1) 293 treated with UV and Untreated lysates; (2) Untreated using Phospho-Chk2 (Thr68) antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。