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It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200

Immunocytochemistry

Immunocytochemistry analysis of Ku70 (green) in CEM using Ku70 antibody,and DAPI(blue).

Western

Western blot analysis of Ku70 in Hela, A549, MCF-7, U251 lysates using Ku70 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Ku70 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。